Effects of intrauterine devices on proteins in the uterine lavage fluid of mares

Intrauterine devices block luteolysis in cyclic mares, but the underlying mechanism is unknown. To clarify mechanisms, protein profile of endometrial secretome was analyzed using two-dimensional difference gel electrophoresis (2D-DIGE). Twenty-seven mares were classified according to whether they inseminated (AI) or had an intrauterine device (IUD), a water-filled plastic sphere, inserted into uterus on Day 3 after ovulation. Uterine lavage fluids collected 15 from pregnant (AI-P; n = 8), non-pregnant (AI-N; 4), and with IUD (n 15). The group further divided prolonged (IUD-P; 7) normal luteal phase (IUD-N; 8) groups basis ultrasound examinations, serum levels progesterone PGFM Days 14 15, COX-2 results 15. Four each selected for 2D-DIGE analyses. Ten proteins significantly different abundance among groups, nine identified. Malate dehydrogenase 1, increased sodium tolerance aldehyde 1A1, prostaglandin reductase albumin hemoglobin highest mares; T-complex 1 annexin A1 6-phosphogluconolactonase mares. suggest that behind likely related inflammation.